Monitoring the Formation of Polymer Nanoparticles with Fluorescent Molecular Rotors. Sascha Schmitt, Galit Renzer, Jennifer Benrath, Andreas Best, Shuai Jiang, Katharina Landfester, Hans-Jürgen Butt, Roberto Simonutti, Daniel Crespy, Kaloian Koynov.Recent Developments in G-Quadruplex Binding Ligands and Specific Beacons on Smart Fluorescent Sensor for Targeting Metal Ions and Biological Analytes. Long Zhao, Farid Ahmed, Yating Zeng, Weiqing Xu, Hai Xiong.The Dual Use of the Pyranine (HPTS) Fluorescent Probe: A Ground-State pH Indicator and an Excited-State Proton Transfer Probe. This article is cited by 259 publications. We further discuss the computational fitting of the model for the nonradiative process of ThT. We discuss the advantages and disadvantages of the various nonradiative models while focusing on the model that was initially proposed by Glasbeek and co-workers for auramine-O to be the best suited for ThT. In the literature, researchers have suggested several models to explain nonradiative processes. This process makes the ThT molecule light up in the presence of amyloid fibrils. The LE state has high oscillator strength that enables radiative excited-state relaxation to the ground state. In the context of biomedical assays, the binding to amyloid fibrils inhibits the internal rotation of the molecular segments and as a result, the electron cannot cross into the nonradiative “dark” CT state. Solvent, temperature, and hydrostatic pressure play roles in this process. This Account discusses several factors that can influence the LE-TICT dynamics of the excited state. This twisted-internal-CT (TICT) is responsible for the molecular rotor behavior of ThT. The electronic wave function of the excited state changes from the initial LE state to the CT state as a result of the rotation around a single C–C bond in the middle of the molecule, which connects the benzothiazole moiety to the dimethylanilino ring. Both ab initio quantum-mechanical calculations and experimental evidence have shown that the electronically excited-state surface potential of ThT is composed of two regimes: a locally excited (LE) state and a charge-transfer (CT) state. The simplicity of this type of measurement has made ThT a common fluorescent marker in biomedical research over the last 50 years.Īs a result of the remarkable development in ultrafast spectroscopy measure-ments, researchers have made substantial progress in understanding the photophysical nature of ThT. Upon binding to amyloid fibrils, the steady-state (time-integrated) emission intensity of ThT increases by orders of magnitude. Thioflavin-T (ThT) can bind to amyloid fibrils and is frequently used as a fluo-rescent marker for in vitro biomedical assays of the potency of inhibitors for amyloid-related diseases, such as Alzheimer’s disease, Parkinson’s disease, and amyloidosis.
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